问题描述：
英语翻译
Genetic screen for intercellular gene transfer.
(A) Maps of the plastid genome in Pt-spec:gfp plants and the transgenic locus in Nuc-kan:yfp plants.PpsbA and TpsbA,promoter and terminator from the plastid psbA gene; Prrn,promoter from the plastid rRNA operon; Trps16,terminator from the plastid rps16 gene; Pnos and Tnos,promoter and terminator from the nopaline synthase gene from Agrobacterium tumefaciens; P35S and T35S,promoter and terminator from the cauliflower mosaic virus 35S transcript; LB and RB,left and right borders of the T-DNA region; Eco RV and Xho I,restriction sites used for restriction fragment length polymorphism analysis (fig.S3).
(B) Selection experiments.The grafted stem region was either sectioned (horizontal lines) or directly exposed to selection (bracket).The middle panel shows the arrangement of tissue explants,the right panel a selection plate (right half,stem sections from the graft site; upper left quarter,three stem sections and three leaf explants from Nuc-kan:yfp; lower left quarter,the corresponding explants from Pt-spec:gfp).After 4 weeks on medium with spectinomycin and kanamycin,some explants from the graft site developed growing callus tissue or regenerating shoots (arrows).
(C) Expression and subcellular localization of the fluorescent reporters.The wild type,the two grafting partners,and a YG line were assayed for GFP,chlorophyll (Chl),and YFP fluorescence.
Genetic screen for intercellular gene transfer.
(A) Maps of the plastid genome in Pt-spec:gfp plants and the transgenic locus in Nuc-kan:yfp plants.PpsbA and TpsbA,promoter and terminator from the plastid psbA gene; Prrn,promoter from the plastid rRNA operon; Trps16,terminator from the plastid rps16 gene; Pnos and Tnos,promoter and terminator from the nopaline synthase gene from Agrobacterium tumefaciens; P35S and T35S,promoter and terminator from the cauliflower mosaic virus 35S transcript; LB and RB,left and right borders of the T-DNA region; Eco RV and Xho I,restriction sites used for restriction fragment length polymorphism analysis (fig.S3).
(B) Selection experiments.The grafted stem region was either sectioned (horizontal lines) or directly exposed to selection (bracket).The middle panel shows the arrangement of tissue explants,the right panel a selection plate (right half,stem sections from the graft site; upper left quarter,three stem sections and three leaf explants from Nuc-kan:yfp; lower left quarter,the corresponding explants from Pt-spec:gfp).After 4 weeks on medium with spectinomycin and kanamycin,some explants from the graft site developed growing callus tissue or regenerating shoots (arrows).
(C) Expression and subcellular localization of the fluorescent reporters.The wild type,the two grafting partners,and a YG line were assayed for GFP,chlorophyll (Chl),and YFP fluorescence.
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